染料法 qPCR 預(yù)混液-AQ601

  產(chǎn)品名稱  

PerfectStart^? Green qPCR SuperMix (AQ601)

  應(yīng)用專利  

專利號：ZL202010542947.0    ZL202010543186.0

  高分文獻集錦  

?Zhang B, He P, Lawrence J E G, et al. A human embryonic limb cell atlas resolved in space and time[J]. Nature, 2023.(IF 64.80)

?Zhang J Y, Wang B, Xu H R, et al. Root microbiota regulates tiller number in rice[J]. Cell, 2025.(IF 45.50)

?Zhao K, Xue H, Li G, et al. Pangenome analysis reveals structural variation associated with seed size and weight traits in peanut[J]. Nature Genetics, 2025. (IF 31.80)

?Xiang B, Zhang M, Li K, et al. The epitranscriptional factor PCIF1 orchestrates CD8+ T cell ferroptosis and activation to control antitumor immunity[J]. Nature Immunology, 2025.(IF 27.80)

?Huang L, Wei M, Li H, et al. GP73-dependent regulation of exosome biogenesis promotes colorectal cancer liver metastasis[J]. Molecular Cancer, 2025.(IF 27.70)

?Deng P, Wang Z, Chen J, et al. RAD21 amplification epigenetically suppresses interferon signaling to promote immune evasion in ovarian cancer[J]. The Journal of Clinical Investigation, 2022.(IF 19.46)

?Huang J, Wu C, Kloeber J A, et al. SLFN5-mediated chromatin dynamics sculpt higher-order DNA repair topology[J]. Molecular Cell, 2023.(IF 19.33)

?Liang Y, Wang J, Xu C, et al. Remodeling Collagen Microenvironment in Liver Using a Biomimetic Nano‐Regulator for Reversal of Liver Fibrosis[J]. Advanced Science, 2023.(IF 17.52)

?Liang Y, Zhang J, Xu C, et al. Biomimetic Mineralized CRISPR/Cas RNA Nanoparticles for Efficient Tumor-Specific Multiplex Gene Editing[J]. ACS nano, 2023.(IF 17.10)

?He F, Liu Z, Xu J, et al. Black phosphorus nanosheets suppress oxidative damage of stem cells for improved neurological recovery[J]. Chemical Engineering Journal, 2023.(IF 16.74)

?He F, Cheng K, Qi J, et al. Black phosphorus nanosheets enhance differentiation of neural progenitor cells for improved treatment in spinal cord injury[J]. Chemical Engineering Journal, 2023.(IF 15.10)

?Chen B, Jin K, Dong J, et al. Hypocretin‐1/Hypocretin Receptor 1 Regulates Neuroplasticity and Cognitive Function through Hippocampal Lactate Homeostasis in Depressed Model[J]. Advanced Science, 2024.(IF 14.30)

?Huang C, Jiang T, Pan W, et al. Ubiquitination of NS1 confers differential adaptation of zika virus in mammalian hosts and mosquito vectors[J]. Advanced Science, 2024.(IF 14.30)

?Liang Y, Zhang J, Wang J, et al. Restoring Tumor Cell Immunogenicity Through Ion‐Assisted p53 mRNA Domestication for Enhanced In Situ Cancer Vaccination Effect[J]. Advanced Science, 2025(IF 14.30)

?Cui B, Guo X, Zhou W, et al. Exercise alleviates neovascular age-related macular degeneration by inhibiting AIM2 inflammasome in myeloid cells[J]. Metabolism, 2023.(IF 13.93)

?Sun D, Zhang X, Yin Z, et al. As-hyperaccumulator Pteris vittata and non-hyperaccumulator Pteris ensiformis under low As-exposure: Transcriptome analysis and implication for As hyperaccumulation[J]. Journal of Hazardous Materials, 2023.(IF 13.60)

  產(chǎn)品特點  

? 3 種抗體封閉，特異性高，靈敏度高，擴增效率強，適用物種范圍廣。

? 雙陽離子緩沖液，增強特異性，減少引物二聚體形成，數(shù)據(jù)準(zhǔn)確。

? 配有適用于不同機型的 Universal Passive Reference Dye ( 調(diào)整 PCR 加樣誤差引起的管間差異 )，校正孔間信號誤差。

  數(shù)據(jù)展示  

擴增效率高

以梯度稀釋的質(zhì)粒 DNA (10 ng-0.1 pg，10 倍稀釋 ) 為模板進行擴增得到的擴增曲線和標(biāo)準(zhǔn)曲線。結(jié)果顯示，TransGen 產(chǎn)品擴增效率高。

不同物種模板擴增

以不同物種的 RNA 反轉(zhuǎn)錄 (TransGen, AT311) 后得到的 cDNA 為模板分別使用TransGen 與 Company T 的產(chǎn)品進行擴增 (NTC 無擴增 )。結(jié)果顯示，TransGen產(chǎn)品擴增效果與 Company T 產(chǎn)品基本一致。

擴增靈敏度

以 500 ng 的人源 RNA 反轉(zhuǎn)錄 (TransGen, AT311) 后得到的 cDNA 為模板梯度稀釋，分別使用 TransGen 與 Company T產(chǎn)品擴增 β-actin (NTC無擴增)。結(jié)果顯示，TransGen 產(chǎn)品擴增靈敏度與 Company T 產(chǎn)品基本一致。

  產(chǎn)品信息