TransScript All-in-One 反轉(zhuǎn)錄試劑—AT341

  產(chǎn)品名稱  

TransScript^? All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal) (AT341)

  應(yīng)用專利  

專利號：ZL 201710031139.6

  高分文獻(xiàn)集錦  

?Yuan F, Cai J, Wu J, et al. Z-DNA binding protein 1 promotes heatstroke-induced cell death[J]. Science, 2022.(IF 63.71) 

?Feng C, Chen B, Hofer J, et al. Genomic and genetic insights into Mendel’s pea genes[J]. Nature, 2025.(IF 48.50)

?Wu H, Qu X, Dong Z, et al. WUSCHEL triggers innate antiviral immunity in plant stem cells[J]. Science, 2020.(IF 41.84)

?Han X, Wang R, Zhou Y, et al. Mapping the mouse cell atlas by microwell-seq[J]. Cell, 2018.(IF 31.40)

?Huang Y, Ji Z, Tao Y, et al. Improving rice nitrogen-use efficiency by modulating a novel monouniquitination machinery for optimal root  plasticity response to nitrogen[J]. Nature Plants, 2023.(IF 18.00)

?Yu Z, Deng P, Chen Y, et al. Inhibition of the PLK-coupled cell cycle machinery overcomes resistance to oxaliplatin in colorectal  cancer[J]. Advanced Science, 2021.(IF 16.80)

?Chen Y, Liu J, Kang S, et al. A palisade-shaped membrane reservoir is required for rapid ring cell inflation in Drechslerella dactyloides[J]. Nature communications, 2023.(IF 16.60)

?Ji S, Luo Y, Cai Q, et al. LC domain-mediated coalescence is essential for Otu enzymatic activity to extend Drosophila lifespan[J]. Molecular cell, 2019.(IF 14.20)

?Zhang Y, Gao Y, Wang H L, et al. Verticillium dahliae secretory effector PevD1 induces leaf senescence by promoting ORE1-mediated ethylene biosynthesis[J]. Molecular plant, 2021.(IF 13.16)

?Sun X, Peng X, Cao Y, et al. ADNP promotes neural differentiation by modulating Wnt/β-catenin signaling[J]. Nature communications, 2020.(IF 12.12)

?Zhang Y, Tan S, Gao Y, et al. CLE42 delays leaf senescence by antagonizing ethylene pathway in Arabidopsis[J]. New Phytologist, 2022.(IF 10.32)

?Gao Y, Li Z, Yang C, et al. Pseudomonas syringae activates ZAT18 to inhibit salicylic acid accumulation by repressing EDS1 transcription  for bacterial infection[J]. New Phytologist, 2022.(IF 10.15)

?Zheng Z, Zhang X, Liu J, et al. GABAergic synapses suppress intestinal innate immunity via insulin signaling in Caenorhabditis elegans[J]. Proceedings of the National Academy of Sciences, 2021.(IF 9.50)

?Yu Z, Deng P, Chen Y, et al. Pharmacological modulation of RB1 activity mitigates resistance to neoadjuvant chemotherapy in locally  advanced rectal cancer[J]. Proceedings of the National Academy of Sciences, 2024.(IF 9.40)

?Zeng Y H, Cai Z H, Zhu J M, et al. Two hierarchical LuxR-LuxI type quorum sensing systems in Novosphingobium activate microcystin  degradation through transcriptional regulation of the mlr pathway[J]. Water Research, 2020.(IF 9.13)

?Zhang L, Xu Y, Cheng Z, et al. The EGR1/miR-139/NRF2 axis orchestrates radiosensitivity of non-small-cell lung cancer via ferroptosis[J].  Cancer Letters, 2024.(IF 9.10)

  產(chǎn)品特點(diǎn)  

? All SuperMix 型：只需加入 gDNA Remover、模板 RNA 和水，實(shí)現(xiàn) cDNA 合成和去除基因組 DNA 同時(shí)進(jìn)行。 

? 優(yōu)化的 Oligo(dT)18 Primer 和 Random Primer(N9) 配比，及 SuperMix 組成，確保對不同濃度的 RNA 有相同的反轉(zhuǎn)錄效率，短鏈 cDNA 合 成效率高。

? 反轉(zhuǎn)錄僅需 15 分鐘。 

? 與 qPCR 試劑的高兼容性。 

? cDNA 只適用于 qPCR，不適用于常規(guī) PCR。

  數(shù)據(jù)展示  

擴(kuò)增效率高

以梯度稀釋（1 μg-100 pg，10 倍稀釋）的不同物種 RNA 為模板，使用 TransGen 和 Company V 產(chǎn)品進(jìn)行反轉(zhuǎn)錄并進(jìn)行 qPCR（AQ601）檢測，結(jié)果表明， TransGen 產(chǎn)品擴(kuò)增效率高。

  產(chǎn)品信息