產(chǎn)品詳情介紹
白介素-12（Interleukin 12， IL-12），又稱為自然殺傷細(xì)胞刺激因子(NKSF)[1]、細(xì)胞毒性T細(xì)胞成熟因子(CLMF)[2-3]或p70 [4]，是由IL-12B(p40)和IL-12A(p35)亞基組成，兩個亞基間通過二硫鍵連接。IL-12是一種重要的免疫調(diào)節(jié)細(xì)胞因子，主要由活化的巨噬細(xì)胞和樹突細(xì)胞產(chǎn)生。在其眾多多效性功能中，IL-12已被證明可誘導(dǎo)IFN-γ的產(chǎn)生、TH1分化、以及T細(xì)胞的活化和NK細(xì)胞的增殖。這些功能是通過IL-12與其異二聚體跨膜受體復(fù)合物結(jié)合介導(dǎo)的 [5]。本產(chǎn)品為融合表達(dá)的p40和p35雙亞基，具有良好的生物學(xué)活性。
[1] Wolf SF, Temple PA, Kobayashi M, Young D, et al. Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J Immunol. 1991, 146(9):3074-81.
[2] Stern AS, Podlaski FJ, Hulmes JD, Pan YC, et al. Purification to homogeneity and partial characterization of cytotoxic lymphocyte maturation factor from human B-lymphoblastoid cells. Proc Natl Acad Sci, 1990, 87(17):6808-12.
[3] Gubler U, Chua AO, Schoenhaut DS, Dwyer CM, et al. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc Natl Acad Sci USA, 1991, 88(10):4143-7.
[4] Kobayashi M, Fitz L, Ryan M, Hewick RM, , et al. Identification and purification of natural killer cell stimulatory factor (NKSF), a cytokine with multiple biologic effects on human lymphocytes. J Exp Med, 1989, 170(3):827-45.
[5] Jayanthi S, Koppolu Bp, Smith SG, Jalah R, et al. Efficient production and purification of recombinant human interleukin-12 (IL-12) overexpressed in mammalian cells without affinity tag. Protein Expr Purif, 2014, 102:76-84.
